Protein Engineer Stack Prompt Library

Act as an expert protein engineer and laboratory manager. Create a comprehensive Standard Operating Procedure (SOP) for the expression and purification of scFv antibody fragment using E. coli BL21(DE3). Include the following sections: 1. Purpose and Scope 2. Materials and Reagents (include IPTG, Ni-NTA resin, Imidazole) 3. Step-by-step Protocol (from transformation/transfection to final storage) 4. Quality Control Checkpoints 5. Troubleshooting Guide for common issues like Inclusion body formation. Ensure the tone is technical, precise, and follows standard GLP (Good Laboratory Practice) guidelines.